Germans and the Holocaust - 2294 Words

German citizens responsible for success of Holocaust Beginning when Adolf Hitler became chancellor of Germany in late January of 1933 and concluding with the official end of World War II in May of 1945, the Holocaust was a period when Jews residing in the German Empire and German-occupied territories were persecuted and harshly murdered. The individuals of the Third Reich were not the first to have anti-Semitic prejudices; however, they were the first to take this type of racism and accomplish massacres on such a grand-scale. The successful killing of approximately six million Jews during the Holocaust can be best explained through the actions of ordinary German citizens as a result of convincing propaganda. It is important to analyze the scope of the intense racism during the Holocaust before examining the actions of German citizens; the Germans were able to cause a great amount of damage to twentieth century Jews, especially in Europe. By the time Hitler won the Reichstag election, there were over nine million Jews living in Europe and most of them resided in countries that Hitler would influence over the next decade. The violence towards Jews began as soon as Hitler came to power. Hitlers bigotries of the Jewish race were no secret, so common German citizens felt comfortable enough to create public disturbances the day after Hitler was elected leader. For example, German individuals attacked Jewish stores without an order from Hitler or his agents, and continued to doShow MoreRelatedGerman History: The Holocaust2588 Words   |  11 Pagesmade a huge impact was the Holocaust. The Holocaust refers to the period January 30, 1933 when Adolf Hitler became chancellor of Germany. Then in M ay 8th, 1945, the war officially ended. It all started after its defeat in World War I, Germany was humiliated by the Versailles Treaty, which reduced its prewar territory, drastically reduced its armed forces, demanded the recognition of its guilt for the war, and stipulated it pay reparations to the allied powers. â€Å"With the German Empire destroyed, a newRead MoreThe Holocaust: German National Pride1027 Words   |  5 PagesNational pride was a key factor in the German people’s indifference to or participation in state-sponsored genocide and murder. There are five main reasons why. Jews were among those blamed by German military officers looking for excuses as to why Germany was defeated in WWI, and thus were linked to the loss of national pride. Jews were seen as bringing down the economy by taking up space and manipulating other Germans into giving the Jews their money to fuel the Jews’ inherent greed. Hitler hadRead MoreRole of Ordinary Germans in the Holoca ust2313 Words   |  10 PagesThe role of ordinary Germans in the Holocaust is that of bystanders. The people of Germany watched on, without protest, as the Jewish people were murdered. Small and large jobs such as engineering and railway work contributed to the operation of the Holocaust and the murdering of Jews. The manipulation of the German people, through racist and anti-Semitic propaganda, speeches and polices from Hitler, meant that millions of Germans backed the plans to rid the nation of Jews. The idea that the JewsRead MoreGerman Jews During The Holocaust1869 Words   |  8 PagesSiyu Song Dr Arms Valaries English 307 German Jews During The Holocaust When the Nazis settled that the Jews were the primary cause of Germany’s problems in the Second World War, they launched a mission aimed at torturing and killing them (Rosenfield 28). In particular, they sought to wipe all the Jews out of the surface of the earth. To gain political mileage, Hitler faulted the Jews for Germany’s economic woes following the First World War. This further created a lot of negative feelings requiredRead More Ordinary Germans and the Holocaust Essay example1713 Words   |  7 PagesOrdinary Germans and the Holocaust Synopsis – Hitler’s Willing Executioners is a work that may change our understanding of the Holocaust and of Germany during the Nazi period. Daniel Goldhagen has revisited a question that history has come to treat as settled, and his researches have led him to the inescapable conclusion that none of the established answers holds true. Drawing on materials either unexplored or neglected by previous scholars, Goldhagen presents new evidence to show that many beliefsRead MoreBlack Germans During the Holocaust Essay1160 Words   |  5 Pagesof Being a Black German during the Holocaust Between 1933 and 1945 Hitler and his minions tortured non-Aryans because they were considered of lesser value to his regime. History books are filled with horrific tales of mistreatment of human beings he considered inferior: Jews, those who were disabled, gay or lesbians. However, most people are unaware that there was a small population of Germans of African descent who were also victims. According to The United States Holocaust Memorial Museum websiteRead MoreEssay about German Nazi: The Holocaust2608 Words   |  11 Pages Final Problem in Germany Germany had many huge problems throughout their years as a country, but one major problem that made a huge impact was the Holocaust. The Holocaust refers to a time period where Adolf Hitlers becomes chancellor and there’s a mass killing in the certain minorities Hitler found responsible for his losses. Hitler mainly looked at the Jews responsible for his losses which led to a mass killing andRead MoreOrdinary Germans and Soldiers are also Guilty for the Holocaust666 Words   |  3 PagesOrdinary Germans and soldiers are culpable in the Holocaust in addition to Hitler and the Nazi inner circle. They are guilty for the Holocaust as well because of their long-standing antisemitism, knowledge of the enormities, and actual assistance in the Holocaust. Although Hitler was the leading force for the Holocaust, he was supported by ordinary Germans and soldiers who agreed with his beliefs and participated in the atrocities just as much. Antisemitism was the beginning and the cause of theRead MoreWhy Common Germans Took Part Of The Holocaust2222 Words   |  9 PagesThis investigation evaluates why common Germans took part in the Holocaust. In order to assess why common Germans took part in the Holocaust the investigation focuses on the participation and complacency of the German people during the Holocaust, specifically the extermination of the Jewish people, and the reasoning behind it. Different explanations for the German actions developed by a range of historians will be presented. The conventional reasons, like psychological and cultural, and the nonconventionalRead MoreWas German ‘Eliminationist Anti-Semitism Responsible for the Holocaust?832 Words   |  3 Pagesâ€Å"Was German ‘Eliminationist Anti-Semitism† Responsible for the Holocaust?† is a fascinating and somewhat discouraging debate that explores the question of whether German anti-Semitism, instilled within citizens outside of the Nazi Party, played a vast role in the extermination of Jews during the Holocaust . Daniel Jonah Goldhagen, author of â€Å"The Paradigm Challenged,† believes that it did; and argues quite convincingly that ordinary German citizens were duplicitous either by their actions or inactions

Psychology Perspectives Columbine Massacre - 1537 Words

Perspectives of Psychology: Understanding the Columbine Massacre In Psychology, there are perspectives and approaches that are looked into when trying to understand how the intricate human mind works. These perspectives are respectfully derived from different ideas and time periods, exemplifying different ways of thinking. These perspectives include: sociocultural, biopsychological, psychodynamic, behaviorism, cognitive, and humanism. These approaches are critically essential in solving something as serious as murder, or simply even why someone acts the way they do. There are many instances where there will be shocking news stories about people committing murders—people that are so unexpected to do such harm. However, when the six†¦show more content†¦The next theory is categorized as a less-modern perspective as it goes all the way back to the Victorian Era. This approach is based on Sigmund Freud’s theory of psychodynamic, which focuses on the role of the unc onscious mind and its influence on conscious behavior, early childhood experiences, development of sense of self, and other motivations. (Ciccarelli, 2012). In other words, this theory states that humans have an unconscious mind in which we repress all of our threatening urges and desires into. In effect of repressing urges, it creates nervous disorders. This approach greatly stresses the importance of early childhood experiences. I firmly believe that both boys, especially Klebold, repressed their urges and thoughts which created a dramatic lash-out. According to New York Times, Klebold had come from a very well educated family that had very high expectations for him. His childhood had been constricted to studying and since he was exposed to such lifestyle at such a young age, he grew accustomed to better his education. Kleboid showed no obvious signs of danger to his family, as he valued how he appeared to his parents. However though, he had a great drinking problem which caused major aggression. Harris, Klebold’s good friend, was greatly interested in creating bombs. His childhood was not like Klebold’s—his family was not as forceful of his studies, or close.Show MoreRelatedArgumentative Essay On Columbine1305 Words   |  6 PagesThe book Columbine by Dave Cullen has been banned for its content about the Columbine School Shooting and the psychology behind the shooting. It was censored from students because of this content that, while being sensitive and triggering to those who are against gun violence or have PTSD from a situation like this, can help spread awareness about school shootings in a world where events such as the Columbine School Shooting are slowly becoming more and more common. This book, having graphic detailsRead MoreEssay on The Ideology of Media Blame2077 Words   |  9 Pagescrime with in society. The Media, particularly television and film have been blamed for many atrocities over the years; some of these can be indirectly related to the medias involvement on peoples lives. But some cannot. As a Media and Psychology student I have different views on the debate of media blame. As a psychologist I can understand the connection with the media and violence, i.e. violence is a behaviour, which in theory, is learnt and repeated on another. ThereforeRead MoreVideo Games Are More Than Half Of The 50 Top Selling Games1666 Words   |  7 Pagesof the 50 top-selling video games contain violence. On April 20, 1999 at Columbine High School in jefferson county colorado, two students and video game fanatics, Eric David Harris, age 18, and Dylan Bennet Klebold, age 17, executed a planned shooting rampage killing 12 other students and a teacher. Researcher and psychiatrist Jerald Block has concluded that Harris and Klebold went on their shooting rampage at Columbine High School after their parents took away their video games, Block arguedRead More The Effects of Media Violence on Children Essay3371 Words   |  14 Pages   Abstract   Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  In recent times, the news media has cried out against violent media, painting it as the leading cause for youth violence. Following events such as the Columbine massacre, news sources have vilified violent media, claiming that it is a primary cause of violent behavior in youths. This analysis provides firm research on the subject from the opposing and supporting sources, giving a thorough definition to the term â€Å"violent media† and  Ã‚  brings forth evidence that other psychologicalRead MoreThe s Theory Of Human Behavior, Emotion, And Thought2450 Words   |  10 Pagesbehavior† (Selterman, 2014). People are driven to form and keep positive and significant relationships with others who reciprocate concern and care. The need to belong motivates human behavior, emotion and thought. Whether looking from a theological perspective, â€Å"But you belong to God†¦Ã¢â‚¬  (John 4:4, English Standard Version) or an evolut ionary view of cell mutations and adaptations, it is inarguable that successful procreation ensures the survivability of mankind. In this act of procreation lies the veryRead MoreThe Effects of Violent Video Games on Society Essay2542 Words   |  11 Pages(11) opened fire on students at a high school in Craighead County, Arkansas as they exited the building for a fire alarm set off by the pair, killing five and wounding ten others. On April 20, 1999, Eric Harris (18) and Dylan Klebold (17) entered Columbine High School in Jefferson County, Colorado and began a shooting rampage that left 13 dead and 21 others wounded. On April 16, 2007, Seung-Hui Cho (23) began a siege on the campus of Virginia Tech that left 32 dead and 17 others wounded. On JulyRead MoreFreedom Of Speech And The Right For Privacy1791 Words   |  8 Pagesmerely joking, which brings up a vital question surrounding the ethical use of the World Wide Web. When is a post or comment protected under â€Å"freedom o f speech,† and at what point has the author of those web posts crossed the line? From a legal perspective, this issue remains confusing. Zane D. Memeger, U.S. Attorney for the Eastern District of Pennsylvania, highlights in his article â€Å"Confronting First Amendement Challenges in Internet Stalking and Threat Cases† the numerous contextual factors thatRead MoreComputer and Internet in Education13526 Words   |  55 PagesHomeNet participant (1995) Kaveri Subrahmanyam, Ph.D., is assistant professor of child development at California State University, Los Angeles. Robert E. Kraut, Ph.D., is professor of social psychology and humancomputer interaction at Carnegie Mellon University. Patricia M. Greenfield, Ph.D., is professor of psychology at the University of California, Los Angeles. Elisheva F. Gross, currently a Ph.D. candidate at the University of California, Los Angeles, was founding creative director of Plug In! TeenRead MoreEvil a Learned Behavior6329 Words    |  26 Pagesbehavior. By exploring ones childhood and family life, its possible to point out potential causes for excessively violent behavior. As well, the society we live in may serve as a catalyst for violence among its habitants. By using anthropology, psychology and sociology it is possible to discover what causes humans to be violent creatures. â€Å"In the human brain, just under the cerebrum stem is the source of aggression and violent behaviors, called the limbic system. This part of the brain is not only

Aboriginal Canadians and European Settlers Free Essays

Aboriginal Canadians and European settlers In the history of contact between Aboriginal and non-Aboriginal peoples in Canada, there has been an imbalance in acculturative influences. Generally, Aboriginal peoples have been changed substantially, with serious erosion of their cultures and identities. However, this dominance by Euro Canadian peoples has also been met by resistance by Aboriginal peoples. We will write a custom essay sample on Aboriginal Canadians and European Settlers or any similar topic only for you Order Now Policy and programme changes to alter the relationship between these two sets of people are suggested, including a reduction in pressures toward assimilation and segregation which have historically resulted in the marginalization of Aboriginal Peoples in Canada. When individuals experience intercultural contact, the issue of who they are comes to the fore. Prior to major contact, this question is hardly an issue; people routinely and naturally think of themselves as part of their cultural community, and usually value this attachment in positive terms. Of course, other life transitions (such as adolescence) can lead people to wonder, and even doubt, which they are. But it is only during intercultural contact that their cultural identity may become a matter of concern. The Royal Commission on Aboriginal Peoples established a research project on Aboriginal cultural identity, and commissioned reports on the subject. This paper is based on one of those reports, and draws upon concepts, data and analyses that were carried out as a consultant to that project The main line of argument in this paper is that intercultural contact between Aboriginal and non-Aboriginal peoples in Canada (both historically, and at the present time), has initiated a process of acculturation (at both the cultural and psychological levels), during which Aboriginal peoples have experienced cultural disruption, leading to reducedwell-being and to identity confusion and loss. It is further argued that sincethis process has resulted from interactions between Aboriginal and nonAboriginal peoples, the key to reestablishing a sense of well-being and secure cultural identity resides in restructuring the relationships between these two communities. This paper contains four sections: a discussion of the concept of cultural identity, as it derives from the social science literature; a brief review of the process and consequences of intercultural contact; a summary of the main findings; and a discussion of their implications for policy and programmed. May lead to more positive identities, and to cultural and psychological outcomes that are more fulfilling. Breton and Norman fishermen came into contact with the Algonquians of the northeast at the beginning of the 16th century, if not earlier, as they put into natural harbors and bays to seek shelter from storms and to replenish water and food supplies. There is some indication that these first contacts with Aboriginal inhabitants were not always friendly. A few individuals were kidnapped and taken to France to be paraded at the court and in public on state and religious occasions. Also, precautions seem to have been taken to hide the women inland when parties landed from ships engaged in cod fishing or walrus hunting. On the other hand, there were mutually satisfactory encounters as trade took place. The Algonquian brought furs, hides and fish in exchange for beads, mirrors and other European goods of aesthetic and perhaps spiritual value. Both sides seemed content with this growing exchange. Soon the Algonquian exacted goods of more materialistic value, such as needles, knives, kettles or woven cloth, while the French displayed an insatiable desire for well-worn beaver cloaks. In the 16th century, the French, like their western European neighbors, proceeded to lay claim to lands â€Å"not possessed by any other Christian prince† based on the European legal theory of Terra Nullius. This theory argued that since these lands were uninhabited, or at least uncultivated, they needed to be brought under Christian dominion. The royal commission to ROBERVAL for the St Lawrence region, dated 15 January 1541, and La Roche’s commission for SABLE ISLAND in 1598 enjoined acquisition either by voluntary cession or conquest. By the early 17th century, as the FUR TRADE expanded and Catholic missionary work was seriously contemplated, a policy of pacification emerged. The fact that the French chose to colonize along the Bay of Fundy marshlands and the St Lawrence Valley, from which the original Iroquoians had disappeared by 1580, meant that no Aboriginal peoples were displaced to make way for colonists. This peaceful cohabitation remained characteristic of Aboriginal-French relations up to the fall of ACADIA (1710) and of NEW FRANCE (1760). Beyond the Acadian farmlands and the Laurentian seigniorial tract, the Aboriginal peoples on their ancestral lands continued to be fully independent, following their traditional lifestyle and customs. Royal instructions to Governor Corellas in 1665 emphasized â€Å"the officers, soldiers and all His Majesty’s adult subjects treat the Indians with kindness, justice and equity, without ever causing them any hurt or violence. Furthermore, it was ordered that no one was to â€Å"take the lands on which they are living under pretext that it would be better and more suitable if they were French. † Royal instructions in 1716 not only required peaceful relations with the Aboriginal peoples in the interests of trade and missions but also forbade the French from clearing land and settling west of the Montreal region seigneurs. In the PAYS Dà ¢â‚¬â„¢EN HAUT, care was taken to obtain permission from the Aboriginals before establishing a trading post, fort, mission station or small agricultural community such as Detroit or in the Illinois country. Following a conference with 80 Iroquois delegates at Quebec in the autumn of 1748, Governor La Galissoniere and Intendant Bigot reaffirmed that â€Å"these Indians claim to be and in effect are independent of all nations, and their lands incontestably belong to them. † Nevertheless, France continued to assert its sovereignty and to speak for the â€Å"allied nations† at the international level. This sovereignty was exercised against European rivals through the allied â€Å"nations,† not at their expense through the suppression of local customs and independence. The Aboriginal peoples accepted this protectorate because it offered them external support while permitting them to govern themselves and pursue their traditional ways. The MI’KMAQ, and later the Arenac, accepted the Catholic religion, even in the absence of large-scale sustained evangelization, as a confirmation of their alliance and brotherhood with the French and resistance to Anglo-American incursions. When the Milkman eventually signed a treaty of peace and friendship with the British authorities at Halifax in 1752, the ABENAKI who had taken refuge in Canada rebuffed the official delegate of the governor at Boston. Beginning their apostolic labors in Acadia in 1611 and in Canada in 1615, Catholic MISSIONARIES dreamed of a rapid conversion of Aboriginal peoples and even wondered if they might not be descendants of the Ten Lost Tribes of Israel. Traditional Milkman and Montagnais hospitality dictated that the itinerant missionaries be well received. Soon evangelization efforts were centered on the sedentary, horticultural and strategically located HURON confederacy (see STE MARIE AMONG THE HURONS). But factionalism arising out of favoritism shown to converts and the EPIDEMICS that decimated the population almost brought the mission to a close. On two occasions, the JESUITS were spared execution or exile on charges of witchcraft only by French threats to cut off the trade on which the Huron had become dependent. Following the dispersal of the Huron in 1648-49, the missionaries turned to other groups in the Great Lakes basin, including the IROQUOIS confederacy, but they never enjoyed great success. Aboriginal peoples assumed a tolerant dualism: â€Å"you can have your ways and we will have ours, for everyone values his own wares. More success was achieved on the reductions, or reserves (see INDIAN RESERVE) as they came to be known, established within the seigniorial tract of New France. In 1637 the seigneur of SILLERY near Quebec was designated a reduction for some Montagnais encamped nearby as well as for all the northern hunters who would take up agriculture under Jesuit tutel age. Although the Montagnais did not remain long, some Arenac refugees came to settle, and finally Huron who escaped from the Iroquois conquest of their country. Eventually there were reserves near each of the three French bridgeheads of settlement: Loretta near Quebec for the Huron; Betancourt and Saint-Francois near Trois-Rivieres for the Abenaki; Kahnawake near Montreal for the Iroquois and Lac-des-Deux-Montagnes for both ALGONQUINS and Iroquois. These reserves were relocated from time to time at ever greater distances from the principal towns not only because of soil exhaustion but also because of the desire of the missionaries to isolate the Aboriginal converts from the temptations of alcohol, prostitution and gambling. The Kahnawake reserve, with the connivance of certain Montreal merchants, became an important link in an illicit trade with Albany and New York. The French designated those Aboriginal peoples who settled on these reserves under the supervision of Missionaries as â€Å"Indiens domicilies† (resident Indians). Those who came to live on reserves were motivated by religious ideals and the need to escape persecution or encroachment on their lands, but in time the economic advantages became quite substantial. It was often on the reserves that canoemen, scouts and warriors were recruited for trade and war. The products of the field and the hunt, as well as the manufacture of canoes, snowshoes and moccasins found a good outlet on the Quebec market. At the time of the British CONQUEST of New France in 1760, the â€Å"resident Indians’ were united in a federation known as the SEVEN NATIONS of Canada. It is possible that this Aboriginal political organization, whose membership evolved over the years, dates back to the early days of the French regime at the time when the first Aboriginal â€Å"reductions† (reserves) were created in the St Lawrence valley. Official French objectives had been to christianize and francize the Aboriginal peoples in order to attain the utopian ideal of â€Å"one people. † The church tried to achieve this objective through itinerant missions, education of an Aboriginal elite in France, reserves and boarding schools, but in the end it was clear that the Aboriginal peoples would not abandon their culture, even when converted. It was the missionaries who, like the fur traders, learned the Aboriginal languages and adopted Aboriginal survival techniques. Racial intermarriage, or metissage, traced its origin to the casual encounters, almost exclusively between Aboriginal women and Frenchmen deprived of European spouses, beginning with the fishermen and sailors along the Atlantic seaboard, and spreading into the hinterland as traders and interpreters, later unlicensed COUREURS DE BOIS, and finally garrison troops came into contact with the interior communities. VOYAGEURS and canoemen travelling to and from the upper country of Canada in the interests of the fur trade acquired the services of Aboriginal women to make and break camp, cook, carry baggage and serve as mistresses. Many of these unions became long-lasting and were recognized locally as legitimate a la facon du pays. Canon law forbade the marriage of Catholics with pagans, so missionaries often had to instruct and baptize adults and children and then regularize such unions. In 1735 Louis XV forbade most mixed marriages; nevertheless the rise of METIS communities in the Great Lakes basin, particularly along Lake Superior, indicated the prevalence of the practice. Warfare was an aspect of Aboriginal life in which the French soon became involved. Most of the Aboriginal people remained steadfastly attached and loyal to France through to PONTIAC’s rising in 1763, with the exception of the Iroquois, Fox and Sioux. Champlain, by supporting his Algonquian and Huron trading partners in 1609, earned the long-lasting enmity of the Iroquois. The French were unable to save the Huron from destruction at the hands of the Iroquois in 1648-49, nor were they able to stop Iroquois incursions into their own or their western allies’ territories until the peace of Montreal in 1701 (see IROQUOIS WARS). The Fox became hostile in 1712 and were the objects of several military expeditions before their dispersal in 1730. The Sioux also often attacked France’s trading partners and allies before agreeing to a general peace settlement in 1754. Canadian militiamen and Aboriginal auxiliaries distinguished themselves also in expeditions to aid Louisiana against the Chickasaws and the Natchez. The escalation of tensions between the French and English over control of the fur trade in North America led to the signing of the TREATY OF UTRECHT in 1713. Under the terms of the treaty, France retained access to Cape Breton Island, the St Lawrence Islands and fishing rights off Newfoundland but ceded Acadia (Nova Scotia) to the British and recognized British jurisdiction over the northern territory of RUPERT’S LAND and the island of Newfoundland. The Mi’kmaq, MALISEET and Passamaquoddy of the area, considered themselves to be friends and allies and not subjects of the French Crown, as well as the rightful owners of the territory ceded to the British Crown. The lack of consultation regarding the terms of the treaty, and the lack of compensation provided to the Mi’kmaq, Maliseet and Passamaquoddy upset them greatly, significantly shifting the balance of power and Aboriginal-French relations in the area. France spent large sums of money for the annual distribution of the â€Å"King’s presents† to the allied nations. In addition, the Crown issued clothing, weapons and ammunition to Aboriginal auxiliaries, paid for their services, and maintained their families when the men were on active duty. These Aboriginal warriors were judged invaluable for guiding, scouting and surprise raiding parties. Their war aims and practices, including scalping and platform torture, were not interfered with as they generally fought alongside the French as independent auxiliaries. In defeat, the French remembered them, obtaining in the terms of capitulation (1760) that they be treated as soldiers under arms, that they â€Å"be maintained in the Lands they inhabit,† and that they enjoy freedom of religion and keep their missionaries. These terms were further reiterated in the Treaty of Oswegatchie, negotiated by Sir William JOHNSON, at Fort Levis (near present-day Ogdensburg, New York), on 30 August 1760, and reaffirmed at Kahnawake on 15-16 September 1760. These two treaties set out the terms for British protection of the interest of the Seven Nations and for the peaceful colonial occupation of their lands. As a result of this conquest, the French monarchy capitulated New France to Great Britain and on 10 February 1763, France and Great Britain signed the TREATY OF PARIS. The treaty outlined the conditions of the capitulation, which involved a series of land exchanges in which France handed over their control of New France to the English. Article 4 of the treaty provided for the transfer of French control of lands in North America east of the Mississippi River to Great Britain. Under the terms of the treaty, Great Britain also gained control of Florida from the Spanish, who took control of New Orleans and the Louisiana territory west of the Mississippi River from the French. In order to establish jurisdiction in the newly conquered Canadian colonies, on 7 October 1763, King George III and the British Imperial Government issued a Royal Proclamation outlining the management of the colonies. Of particular importance, the proclamation reserved a large tract of unceded territory, not including the lands reserved for the Hudson’s Bay Company, east of the Mississippi River as â€Å"hunting grounds† for Aboriginal peoples. As well, the proclamation established the requirements for the transfer of Aboriginal title to the Crown, indicating that the Crown could only purchase Aboriginal lands and that such purchases had to be unanimously approved by a council of Aboriginal people. The proclamation also provided the terms for the establishment of colonial governments in Quebec, West Florida, East Florida and Grenada. The colonies were granted the ability to elect general assemblies under a royally appointed governor and high council, with the power to create laws and ordinances, as well as establish civil and criminal courts specific to the area and in agreement with British and colonial laws. References: 1994 Aboriginal Cultural Identity. Report submitted to the Royal Commission on Aboriginal Peoples. Ottawa, Ontario Aboud, F. 1981 Ethnic Self-identity, in R. C. Gardner and R. Kalin (Editors): A Canadian Social Psychology of Ethnic Relations. Toronto: Methuen. Keefe, S. E. 1992 Ethnic Identity: The Domain of Perceptions of, and Attachment to Ethnic Groups and Cultures. Human Organization 51 :35-43. How to cite Aboriginal Canadians and European Settlers, Essay examples

Law of Business Organization Capital Maintenance Doctrine

Question: Discuss about the Law of Business Organizationfor Capital Maintenance Doctrine. Answer: The Capital Maintenance Doctrine The capital maintenance doctrine signifies that a limited company is required to keep its capital reserve intact in the best interest of the creditors of the company. The creditors contribute to the capital reserve of the company and their contribution indicates the fact that they are entitled to the repayment of that capital. The reduction in the company capital can reduce the liability of the members and subsequently, the position of the creditors is at risk. The retaining of the capital by the company acts as a guarantee that the creditors shall not be prejudiced. The origin of the doctrine can be traced back in the 19th century. The doctrine was embedded in England and has been enacted through the Companies Act 1985 that has been modified in the Company Act 2006. The Australian legislation has many similarities with the legal framework of several nations such as The United States and The United Kingdom. The capital maintenance doctrine was adopted in the Australian Corporate law after being influenced by the universal application of the principle in the legislation of overseas nations. The doctrine was established in the landmark case of Trevor v. Whitworth where the company bought its own shares and at the time of liquidation of the company, one of its shareholders approached the court for the amount he owed to the company. The court established the rule that the shareholder must be paid and that a company is not permitted to buy its own shares. It was further held in the Aveling Barfords case that when a company winds up, the shareholders of a company should be paid after the payment of the creditors. The capital maintenance rules stipulates: capital reduction; financial assistance; share buy-backs; dividends. The Company Act 2006 states that a company making distributions out of its capital reserves is unlawful. The capital maintenance rules have been incorporated in the Corporations Act 2001 under section 256 A that enables a company to reduce its share capital and buy-back the shares in the best interests of the creditors and the shareholders of the company. The Act stipulates that a company must address any risks involved in its transactions and disclose before the creditors all relevant information to retain a fair balance between the company and its shareholders. The statutory provisions relating to the doctrine have been reformed in the year 1980 in the UK to meet the modern business necessities. The provisions were relaxed to the extent that the company was permitted to buy back or redeem its own shares. In 1998, Australia, the Corporation Act 2001 relaxed the capital maintenance rules under section 256 B where the company was allowed to reduce its capital with due authorization of law. Section 257 A enables the court to buy-back its own shares after the approval of the shareholder and ensuring that the company is able to make payments to its creditors. However, the capital maintenance rules have been subjected to criticisms as it involves time-consuming processes and is expensive. The capital maintenance regime requires further modifications to provide more effective creditor protection.

Digestive System Essays (319 words) - Digestive System, Medicine

Digestive System The digestive system is a alimentary canal; it consist of the mouth, pharynx, esophagus, stomach, small intestines, large intestines and anus. The mouth is the first process of the digestive system. The mouth breaks down the food you consume. You chew the food, which is known as masticating, the food is then mixed with saliva broken down for your body to absorb and use. The food then travels to the esophagus which is connected to the stomach. contractions known as peristalsis, push the food down to the stomach. The stomach is a sac-like organ with strong muscular walls. It is located on the left side of the abdominal cavity. The food enters at the cardioesophageal sphincter. The stomach acts as a storage tank for food. It doesn't only hold the food but it also mixes and grinds the food done by chemical breakdown of proteins . The stomach is the body's major digestive organ, the nutrients are absorbed by the blood in the organ. The processed food is now chime which is then sent to the small intestines. The small intestines consist of three parts, the duodenum, jejunum, and the ileum. The duodenum is attached to the stomach, and curves around the head of the pancreas. The jejunum is attached to the duodenum and the ileum extends from the jejunum to the large intestines. The breakdown of the chyme are mixed with enzymes, and bile enters from the gall bladder. The large intestines then eliminates indigestible food from the body as feces. Though the large intestines does not participate in digestion of food, and to help pass the feces, goblet cells produce mucus to act a lubricant. The large intestines consist of the cecum, the appendix which hangs from the cecum. The large intestines leads to/consist of the colon, rectum, anus. The digestive system is used for the break down

The 8 Best Jobs for Teens and How to Find Yours

The 8 Best Jobs for Teens and How to Find Yours SAT / ACT Prep Online Guides and Tips Looking to gain a little more independence and real-world experience? Hoping to develop some useful professional skills? Getting a job as a teenager is a great way to work towards these goals. In this post, I'll talk a little bit about why you should (or shouldn't) get a job, before telling you what you should be looking for in your first part-time position. Read to the end for tips strategies on finding the best job for you! Why Should You Get a Job as a Teen? Maybe you're still deciding whether you want to jump into the world of employment; maybe you've made up your mind that getting a job is definitely right for you. No matter where you stand, I hope you read this section carefully - if you get a job for the wrong reasons, you might end up being counter-productive in the long run. Many students think it's important to get a job for the sake of their college applications. It's great to demonstrate that you can juggle different responsibilities and that you participate in activities outside of school. Keep in mind that admissions officers would rather see strong academic performances than mediocre performance + a part-time job. For this reason, I think jobs can be really great during the summer when you have a lot of free time - you can gain employment experience while also bringing in your own personal income. Working part-time while school is in session is a different story. Many students do really well in school with a part-time job, but there are more variables to consider. Namely, you have to think about prioritizing your other day-to-day responsibilities: school and homework, extracurricular activities, family, and friends. If you can balance these priorities in addition to a part-time job, go for it! If you're struggling to keep up your grades and other activities, however, you'll benefit much more in the long run by focusing on academics than you would by working part-time. It's important to find that balance between school, work, and fun. One important exception to this reasoning is if you need to get a job for financial or familial reasons. If circumstances necessitate that you work part-time while you're in school, and this negatively impacts your grades, it's important that you explain these circumstances on any college or scholarship applications. Now that we've got that out of the way - on to the fun stuff! What Should You Be Looking For in a Teen Job? Chances are you won't be making a ton of money as a teen in an entry-level position. Everyone has to start somewhere, though, and if you're thoughtful about what sort of job you want, you'll be able to get a lot out of your first employment experience. I've come up with a list of skill development areas that should generalize to many jobs (and other aspects of your life as well). You don't have to look for a job withall of the qualities I'm about to list, but here are some possible job skills benefits to consider. Think about which job qualities are most important to you: Responsibility Will people be depending on you? Is it important that you adhere to a regimented schedule, or that you complete a series of tasks or projects in order for things to function smoothly? If yes, you can expect that a particular job will help you develop a strong sense of responsibility, which is a great quality to develop as a teen. Now, most jobs will require you to demonstrate some degree of responsibility. What will ultimately vary among jobs is what you're supposed to be responsiblefor.Here's a general guide to the responsibility hierarchy that you'll find applies to most jobs: Responsible for listening to directions completing tasks Responsible for yourself - that is, doing your job without being told Responsible for taking initiative - anticipate what needs to be done, complete tasks effectively without explicit direction Responsible for other people - direct, teach, and train others. In most entry level positions, you'll start off at the lower end of this hierarchy. Some people prioritize opportunities to gain more responsibility and autonomy - do you? People Skills People skills development is a great advantage of customer service jobs (think working in a restaurant or retail store).Anyone who’s worked in customer service can tell you that you’ll encounter less-than-pleasant people, but you'll also have awesome one-on-one experiences with great customers. Any future jobs or career options will appreciate that you can work well with people, even (especially) difficult individuals. Teamwork Collaboration Some jobs are pretty solitary - you might be interacting with others in a service capacity (for example, as a checkout clerk), but you won't be collaborating with your coworkers much. Jobs where it is important to develop a sense of community, or where you work with coworkers on projects, help build your teamwork skills. Jobs that emphasize collaboration and community can be especially valuable for your resume and college applications. You'll be hard pressed to find a future employer who doesn't highly value a team player. Leadership Growth Potential You’ll find leadership growth potential in positions where you get more responsibility and/or autonomy once you’ve proven yourself. For example, in a restaurant, there might be opportunities to work your way up from busboy to waiter. In a retail environment, you could move from sales associate to shift manager. This upward trajectory comes from hard work and a demonstration of initiative, and usually accompanies pay raises and a fancier job title. This will help you both in future job searches and in your college applications. Additionally, you'll sure get a healthy dose of self-satisfaction that comes with being rewarded for your hard work. Professional Development This one might be a bit more difficult to find in an entry-level job for teens, but it’s definitely possible. By professional development, I mean developing interests, passions, and network connections based on your future career interests in a particular field. For example, if you’re interested in going to veterinary school, you might seek out work or volunteer positions at local animal shelters. If you’re interested in the hospitality industry, you could look for hostess jobs at local restaurants or front desk positions at hotels or inns. If you’re interested in research, check out labs at local universities for part-time research assistant positions. The possibilities are endless and really depend on your own unique interests and skills. 8 Great Jobs for Teens and Where to Find Them Now that we've gone through important job characteristics, I'll list some ideas for the best jobs for teens, along with corresponding job skills strategies. This list is by no means exhaustive, however, so if you have your own ideas about part-time jobs you'd like, I encourage you to do your own research and check them out! Don't worry if you have to send out many applications before getting a call for an interview; that's pretty typical for a job search. Babysitting Job skills: Responsibility, people skills How to find positions:It's best to start with families you already know. Your business will grow through word of mouth if people are happy with your services. You can also post fliers at schools, churches, and community centers. Animal Shelter Worker Job skills:Responsibility, teamwork collaboration, possible leadership growth potential, possible professional development How to find positions: Visit your local animal shelters or rescues and ask for part-time employment or volunteer applications. Some shelters may require you to be 18 or older, so you may have to ask for parental permission if you're underage. Landscaper Great summer job option, and it tends to pay pretty well (although it's very hard physical work). Job skills: Responsibility, teamwork collaboration, possible leadership growth potential How to find positions: Visit local landscape companies ahead of the busy season (spring/summer) to ask if they're looking for seasonal help. Grocery Store Clerk The great thing about clerk positions is that you can find them almost anywhere. Some larger companies even offer college scholarships (Wegmans, for example). Job skills: Responsibility, people skills, leadership growth potential How to find positions: Check online on stores' websites, or visit their customer service desks to ask for applications. Restaurant Staff Depending on your past restaurant industry experience, you might start off as a busser, waiter, or host. Bussers, in particular, don't have to be experienced, but once you're in you can work your way up the ladder. Hourly wages are really low (below minimum wage), but you can make quite a bit of money in tips depending on where you work. Job skills:Responsibility, people skills, teamwork collaboration, possible professional development How to find positions: Check online for local job postings, or ask for applications directly from restaurants. Sales Associate There are so many different types of sales associate positions; if you're in the right geographical area, it's possible to find something tailored to your interests. Are you passionate about fashion? Check out department stores or local boutiques. Are you active or outdoorsy? Look into sporting goods stores. More interested in tech? Check out computer or cell phone sales. Job skills: Responsibility, people skills, leadership growth development, possible professional development How to find positions: Check online for local job postings, or ask for applications directly from stores. Tutoring This is a great way to hone your own skills while helping someone else with your talents. Job skills: Responsibility, people skills, leadership growth potential How to find positions: Check if there are any tutoring programs offered through your school. Look into local tutoring agencies or volunteer groups. Ask your parents if they have friends with younger kids who could use tutoring help. List yourself on online tutoring platforms like Wyzant. Go Independent With newer online platforms, it's easy for budding entrepreneurs to advertise their wares and services both locally and globally. If you have a particular skill that you're passionate about, you don't have to abandon it to pursue a more typical part-time job. If you can provide a service (for example, maybe you're a graphic designer or programmer), you could likely find independent part-time work. If you're an artist or craftsman, you might be surprised to find that there's high demand for high-quality, customized goods. Job skills: Varies How to find work: Offer services through word of mouth to local businesses, friends, and family. You can also check out online platforms like Elance. If you produce goods, consider attending crafts or art fairs, or making an Etsy or Ebay account. What's Next? Looking to save your hard-earned earnings from your part-time job? Check out our guides to saving money on the SAT and ACT. If you hope to get a job so that you can save up for college, you might want to look into some of our guides to financial aid, including information on the Pell Grant, Direct Subsidized, and Direct Unsubsidized loans. Want to improve your SAT score by 160 points or your ACT score by 4 points?We've written a guide for each test about the top 5 strategies you must be using to have a shot at improving your score. Download it for free now:

Criminal Law and Historical Focus Essay Example | Topics and Well Written Essays - 2500 words

Criminal Law and Historical Focus - Essay Example (Landau, 2002) In presenting England's criminal law, Blackstone a common lawyer of earlier generation, chose not to highlight settled procedural distinctions and arcane terms, but instead he invoked a distinctive kind of legal wrong he identified as "public" in nature. The terms crime and criminal law, while enjoying wide linguistic currency, were not always the part of technical vocabulary of the law. In the medieval period, English law was explicitly differentiating between civil and criminal materials. And as is immediately disclosed by the titles of such works as Lord Kames "History of the criminal law" (1758) and William Eden's "Principles of penal law" (1771), eighteenth-century jurists certainly supposed there existed a general category of law that might serve as the object of their scholarly attention. English law, according to a familiar complaint, simply lacked much by way of system or coherent organization, particularly as compared with Roman law, which hitherto dominated university law studies and which set the relevant standard for juristic elegance. "It was thought impracticable to bring the Laws of England into a Method", explained by one of Blackstone's eighteenth-century precursors, "and therefore a Prejudice was taken up against the study of our Laws, even by Men of Parts and Learning". On this basis, English law along with the Canadian law was unable to become an object of rational learning, and instead had to be mastered through the practical, craft-like techniques of legal apprenticeship. Blackstone, as a lawyer was confident of the English law's credentials as a rational system, but the efforts of other lawyers were not considered "failure" to bring changes in the system after negotiations. Law Reform Law reform was supposed to be the one and only asset, exclusively the preserve of lawyers, and Bentham's combination of the wisdom of the reformer and the craft of the lawyer recognized its potential to divide the legal profession. The common lawyer's innate respect for existing institutions and his appreciation of the subtleties of the status quo not only made novelty in the early years of entering into the criminology, but also it particularize the concept of jurisdiction in the early years of reform, as an object of suspicion. The means and scope of amendment were a further cause for division, even after its need was established. There was a disturbing element of self-examination in law reform to which some lawyers found it difficult to respond with enthusiasm. The status quo would always find friends in influential positions, particularly in respect of criminal law reform amongst the judiciary. Law - A Profession towards Criminal Trial Process As the pace and scale of law reform increased it became an intense preoccupation of the legal profession and for the eighteenth century lawyers, acted as a charm to enter into such a jurisdiciary profession. It was the dominant theme of professional literature of that period that lasted the debate over the role of counsel and advocacy in the criminal trial, for a longer period of time and consideration in this work, could be traced in parliamentary debates, select committee minutes, the evidence and reports of the Criminal Law Commissioners, periodical and professional journals, biographies and other professional lite

Accounting Course - One.Tel Telecommunication Company Essay

Accounting Course - One.Tel Telecommunication Company - Essay Example Finally, by March 2001, One Tel was declared insolvent, after continuous efforts by News Limited and PBL to inject additional cash turned out to be insufficient. In this report, the reasons for the collapse are analyzed, how it could have been prevented and its effect. This paper analyses how the company was mismanaged, its internal control weaknesses, and financial reporting issues. Jodee Rich and Brad Keeling established One Tel. Company (telco), on the 1st of May 1995. James Packer and Lachlan Murdoch were the initial backers, providing an investment worth $995 Million (Cook, 2001). The company’s business was to provide communication services and networks in Australia and major countries of Asia and Europe. The company launched its GSM services network in 1995 and within few years it became the fourth largest telecommunications company of Australia. Its success was mainly fuelled by its marketing and ability to attract young mobile users. It achieved an early success in Australia and very soon its operations went across international boundaries. The marketing strategy was to focus on the younger generation, using the slogan â€Å"You’ll tell your friend about One Tel†. In its early years, One Tel enjoyed a lot of success due to its growing customer base and its profits grew from $3.7 million in 1997 to $6.9 million in 1999. In November 19 99, One Tel. had a market capitalisation of $3.8 billion that made it rank as the 30th largest Australian company (Barry, 2002). Unfortunately, due to gross mismanagement the company’s collapse was inevitable. In 2000, the company’s financial position reported a loss $291.1 million. The shareholders tried to keep the company afloat, but it was not to be and by 28th May 2001 the company was de-listed. The downfall of One Tel can be largely attributed to its shareholders and

FAA inspections on Boeing 737's and how it relates to Operations Research Paper

FAA inspections on Boeing 737's and how it relates to Operations Management - Research Paper Example It provides employment opportunities to over 170,000 individuals throughout 70 nations. It is also regarded as one of the oldest manufacturers of commercial airlines having its root from the last 40 years. It also manufacturers missiles, defense systems and satellites and is a major service provider to NASA (Boeing, 2013). The main aim of this research paper is to provide a broad description on the main reasons behind the Federal Aviation Administration (FAA) to order inspections of more than 1,000 Boeing 737 jets and to show appropriate reasons that the inspection of the airline can help the company in ensuring proper management of operations. Operation Management Operations management is considered as one of the most vital parts of an organizational life cycle irrespective of the fact that it is involved in producing goods or services or is functioning in private or public sectors. The main purpose or aim of operations management is to implement steps that may help the organization to operate efficiently. With reference to the article, â€Å"FAA Orders Inspections on Boeing's 737 Aircraft† by Andy Pasztor, it can be apparently viewed that the potential effect of factory defects in the manufacturing process of Boeing 737 has led the company to inspect more than 1000 Boeing 737s, causing an incurrence of total compliance costs of nearly US$10 million (Pasztor, 2013). The main steps which are usually followed in the process of operations management are discussed below: Contact customer: The first step in operations management is to obtain information on the demand, intension and the core customers/potential customers to buy the products’ produced. Moreover, conserving the contact information is also done to measure the market action (Mahadevan, 2009). Contribute a demand: The next step in the process is to propose a demand of service or product to the qualified customers. This can be done by offering a written proposal to potential customers. It is considered that business proposal is often determined to be a step in settling complex business processes. For instance, companies like Boeing usually adopt the policy of providing business proposals to potential airlines in order to offer them a broad description of the services they render and the way in which they differ from their competitors (Mahadevan, 2009; Neely, 1993). Sale: In this step, the contract or sale of the product is done to their potential customers. Establish a written procedure: In this vital step, both the parties agree upon a mutual agreement in keeping with the different aspects such as characteristics and date of supply of the final products among others. The step is recognized to be a vital part as both the parties are benefited from it (Mahadevan, 2009). Required resources: In this step, all the necessary equipments, products and additional resources needed to complete the project are verified. The costs of the entire requirements are measured to impleme nt it in inventory management (Mahadevan, 2009). Execution: In this phase, the written process is applied in the practical form, resulting in the formation of the defined product (Mahadevan, 2009). Effectiveness: The final expectation according to the contract or customer is measured. Delivery: This step involves the process of delivering the final product to the end customer. This step needs

Hydrolysis of Phosphomonoesters Study

Hydrolysis of Phosphomonoesters Study Introduction Site-directed mutagenesis was the primary basis of this portion of the laboratory directed toward determining the active site of the enzyme catalysis for hydrolysis of phosphomonoesters. The criteria was to turn the original sequence with Arginine 166 into Glutamine 166 through a point mutation. The primer location is located in the 401511 sequence of the phoA gene of alkaline phosphatase in E. Coli. The primer sequence is: 5- G CTG GTG GCA CAT GTG ACC TCG CGC AAA TGC TAC GGT CCG AGC -3’. The mutated primer sequence is: R166Q1 5’-G CTG GTG GCA CAT GTG ACC TCG CAG AAA TGC TAC GGT CCG AGC-3’. The reverse complementary mutated primer sequence is: R166Q2 5’-GCT CGG ACT CGG ACT GTC GCA TTT CTG CGA GGT CAC ATG TGC CAC CAG C-3’. A calculation for the melting temperature can be determined by the following equation: TM= 81.5 + 0.41 (%GC) – 675/N- % mismatch The TM value for Gln-166 was determined to be 85.95ËÅ ¡C. It is necessary for the TM value to be above 75ËÅ ¡C for successful mutation. Running on the null hypothesis, it would be clear that nothing would change for the kinetic parameters. If something were to change, the kinetic parameters for the Michaelis constant (Km), which determine the velocity of an enzyme as well as calculated to be  ½ the Vmax, would be hypothesized much higher after the mutation to glutamine since the mutation allows for 61% more accuracy from arginine’s CGG at 25% to glutamine’s CAG at 86%. The increase in the binding affinity creates the hypothesis that since it is bound closer together creating a stronger binding affinity, the maximal speed (Vmax) would be reduced since it does not require as much conversion between substrate and product. Mechanism of Alkaline Phosphatase Alkaline phosphatase (APase) is an enzyme located in the periplasmic space of E. Coli. The structure of APase, usually in the form of a dimer, shows two zinc metallonzymes and a magnesium ion in the active center. There is a bridging ligand with the protein Asp51 in the active center for the two zinc ions and the one magnesium ion. There are four phosphate oxygens; two phosphate oxygens form a phosphate bridge between the two zinc ions and the other two form hydrogen bonds with the guanidinium group of arg-166 (Coleman, 1992). The dimer is two identical subunits that each contain 429 amino acids. (Coleman, 1992). The most important amino acids located at the active site of APase are the four Cysteine residues represented as combined intrachain disulfides. The phosphorylated residue was Ser 102, which is phosphorylated during phosphate hydrolysis, to begin the nucleophilic attack on phosphorus. Each monomer contains a leucine rich environment of 22 residues. (Coleman, 1992). The first zinc ion is necessary in activating the leaving group of the ester oxygen in order for Ser 102 to phosphorylate (Coleman, 1992). The second zinc ion is necessary for the ester oxygen of the serylphosphate to begin activation of the phosphoseryl intermediate during hydrolysis of Ser 102. When the phosphoseryl intermediate is formed during the first zinc formation, a hydroxide is formed. (Coleman, 1992). Figure 1: this figure represents the monomer of alkaline phosphatase showing the zinc triad with magnesium in the active center. (Coleman, 1992). The proposed mutation as discussed, is mutating the arg-166 to glu-166. It is speculated that there will be no effect on the enzyme during the mutation. Arg-166 is located in the guanidinium group and is an electrophilic species while APase is being phosphorylated. Arg-166 is speculated to play a role in stabilizing the developing negative charge on the oxygen of the leaving group, pentacoordinate transition state, or help bind the phosphate group. As discovered byButler-Ransohoff et al, it is discovered that Arg-166 has no effect on the hydrolysis of phosphate monoesters and therefore will not be effected after mutation. Competent Cells Competent cells are used to help the DNA get into the cell. DNA is negatively charged and the calcium ions from the competent cells of DH5-ÃŽ ± cells are positively charged. The purpose of calcium chloride’s positive ions is to create an equilibrium between negative charge on the cell membranes phospholipid heads and the phosphate group on the plasmid DNA. This is where the genetic modification occurs. Therefore, they help by binding to the DNA and move it into the cell after the cell goes through a heat shock cycle. Competent cells have a very high rate for transformation efficiency. The proposed understanding of how this works, is that the calcium ions are positive and therefore weaken the electrostatic repulsion, which in turn weakens the cell walls. When the cell goes through heat shock, it causes the pressure to increase opening the cell and allowing the competent cells to bring the DNA into the cell by allowing pores to be created with the weakened cell wall. This forces the plasmid to become supercoiled so that it can pass through the pores created by the charge difference and heat shock. The competence comes from the cold bath of DH5-ÃŽ ± cells in calcium chloride which shocks the cells causing pores and then heat the cell to 42ËÅ ¡C for roughly 2 minutes, more than this could cause the cell membrane to denature and the pEK-154 mutated plasmid would become denatured. DH5-ÃŽ ± is a strain from E. Coli commonly used in laboratory practices due to it having the phoA- gene deficiency. This is necessary and desirable because it is easily transformed for creating competent cells. DH5-ÃŽ ± cells are used because they are a common strain in E. Coli and are commonly used for cloning. In addition, they promote stability and help improve the quality of the plasmid when using the Miniprep kit. (Dagert, 1979). A growth curve was created after incubation of the cells for competent cells. This was done to show the growth period during the lag phase is depicted by the highest point on the growth curve. This is taken to determine how much growth you have and is determined through a logarithm vs. time on a graph. The method used is optical density (OD) at 600nm to measure the transmittance on a spectrophotometer. If the value for the OD600 is high, the protection factor by a filter is lower and vise versa. This method is used to determine how much light is absorbed through the bacterial cells. Site-Directed Mutagenesis The sequence chosen for the point mutation from Arginine 166 to Glutamine 166 was sequenced in Ann Arbor. Site-Directed Mutagenesis synthesizes two complimentary oligonucleotides double stranded DNA template with glutamine 166 mutation, which is tagged with unmodified nucleotide sequences to a single stranded DNA template to allow mutation to occur and then reforms the new double strand DNA template during thermal cycling. When this occurs, the newly single stranded DNA template forms complementary strands with the enzymes and nucleotides to produce a higher result of strands. The purpose of site-directed mutagenesis is to make specific changes to the DNA sequence of a gene. Quik Change Lightening Mutagenesis kit provides a faster and more reliable insertion of the mutation with a simple three step method. The multiple cycles are subjected to a polymerase chain reaction (PCR) method. This allows multiple sets of mutated DNA to be produced within a short period of time. PCR works by heating and cooling the samples, since the double DNA is subjected to separation when heated it causes the srtands to separate allowing nicks in the template to occur. pUC-18 is a control plasmid used for determining if a mutation was successful and efficient. Figure 2: pUC-18 plasmid DNA genetically mutated to contain the LacZ gene as well as the ampicillin resistance gene. The polylinker cuts the section of the DNA by the Dpn1 restriction endonuclease creating a linear DNA strand allowing for the binding of the mutation of Arg-166 to Glu-166 to occur. pUC-18 is a circular double stranded DNA molecule. The reason pUC-18 was chosen as the control positive plasmid is because it was genetically manipulated to contain an ampicillin resistance gene as well as a ÃŽ ²-galactosidase enzyme known as LacZ. The lacZ gene is essential for DNA mutation because it contains a region to insert a polylinker, which recognizes the Dpn I restriction endonucleases during digestion causing the plasmid to become linear and bind to the mutated plasmid DNA that has also been cut with the Dpn I restriction endonuclease. A pWhitescript is used as the positive control plasmid used for mutagenesis. The control plasmid is combined with pEK154 plasmid containing the phA gene of APase and the vector double stranded DNA. The two oligonucleotide primers are the opposite ends of the vector. pWhitescript has the stop codon TAA inserted that stops the ÃŽ ²-galactosidase enzyme from producing. The two oligonucleotide control primers create a point mutation that turns the T residue of the stop codon to the C residue of the glutamine codon (Gln, CAG). That allows enzyme ÃŽ ²-galactosidase to be produced after pWhitescript is subjected to mutagenesis. There are two oligonucleotide with the mutation primer as discussed earlier. New DNA polymerase called Q1 enzyme was used for double stranded DNA template when combined with dNTP mix it extended the two-oligonucleotide primers as well as allowing the new plasmid DNA to construct. PCR Cycling parameters were used during Quik Change Site-Directed Mutagenesis for the transformation of arginine to glutamine by the use of the Pfu Enzyme. As visible from table 1, the cycling parameters were set up for this specific reaction for a point mutation. After cycling, the non-mutated parental supercoiled double stranded (dsDNA) is digested. Table 1: Quik Change Site-Directed Mutagenesis Parameters Segment Cycles Temperature Time 1 1 95C 2min 2 18 60C 60C 68C 20 sec 10 sec 30 sec/kb of plasmid length (3min) 3 1 68C 5 min The first step of the three step simple method for Quik Change Lightening Mutagenesis kit is Thermal cycling at 95ËÅ ¡C for two minutes. During this time, the DNA template becomes denatured allowing the primers to be subjected to heat treatments, which denature the pEK-154 template DNA strand and synthesizes the primers to extend linearly and cause nicks with Pfu Fusion-based DNA polymerase, which are then sealed by components within the Pfu enzyme blend. This polymerase allows for exact replication of original template and does not disrupt the orientation. The original mutated pEK-154 was used as the supercoiled double stranded DNA template Figure 1: during thermal cycling, the double-stranded DNA Template is nicked and subjected to point mutation for arginine 166 to glutamine 166 and then sealed with components in the Pfu enzyme blend. In the second step of the kit the template digests the parental DNA strand with methylated and hemimethylated DNA using the enzyme Dpn I endonuclease and subjected to thermal cycling for the second segment. The un-mutated pEK-154 plasmid DNA is the parental DNA while the mutated pEK-154 is the plasmid DNA with the desired mutation. With the nicks, the mutated plasmid with the two oligonucleotide primers is mixed with Dpn I endonuclease for methylated DNA that targets the sequence 5’-Gm6ATC-3’. This sequence helps digest the pEK-154 parental DNA template and has not been introduced into the methylase enyme allowing methylation to not effect the DNA and therefore will not be digested with the parental DNA strand. Similarly, the un-mutated is also digested in Dpn I restriction endonuclease to be methylated. Figure 2: Dpn 1 enzyme endonuclease digests the parental DNA template with methylated and hemimethylated allowing for single stranded DNA for transformation. Step 3 is the last cycling step where transformation of the newly mutated glutamine 166 single-stranded DNA. The DNA is added to Dpn I restriction endonuclease, which forms it into linear DNA stranded DNA duplex forming a double stranded DNA for the newly synthesized mutated DNA. After the mutated vector DNA with the primers has been nicked it is transformed into CaCl2 competent DH5-ÃŽ ± cells. This transformation must occur to repair the nicks caused during cycles to separate the DNA strands. The pWs, which is used as the control DNA is also transformed with competent DH5-ÃŽ ± cells to help repair the nicks. Transformation Control screening used X-gal and IPTG to determine if ÃŽ ²-galactosidase activity. The newly mutated pEK-154 cells are screened for alkaline phosphatase activity. This helps screen for ÃŽ ²-galatosidase enzyme in E. Coli is a Lac Z gene that codes for this enzyme. This enzyme is useful because it breaks the lactose into galactose and glucose. A Lac operon contains an operator and a promoter, which binds RNA-polymerase that starts transcription for the Lac I gene for I protein which can only bind to operator or lactose, but not both. When I protein is bound to lactose, in this case Isopropyl-1-ÃŽ ²-D-galactopyranoside (IPTG) which mimics the structure of lactoase allowing RNA-polymerase to bind to the promoter to produce ÃŽ ²-galactosidase. 5-bromo-4-chloro-3-indolyl-ÃŽ ²-D-galactopyranoside (X-gal), which is a substrate that cleaves galactose in ÃŽ ²-galactosidase to produce a blue color. If no ÃŽ ²-galactosidase is present they turn white on the agar plates. This determines if the cells contain the mutated CAA codon for glutamine instead of the stop codon TAA of the unmutated protein. 5-bromo-4-chloro-3-indolylphosphate (BCIP) is used to determine if alkaline phosphatase is present. BCIP like X-gal is a substrate that cleaves the phosphate group off of alkaline phosphatase producing a blue color.

indias safest cars :: essays research papers

How often do you wake up to the news of a road accident that killed a few people in some part of the country? Almost everyday, right? Then you quickly browse through the other news and life goes on. Every year, over 80,000 people die on Indian roads; every five road accidents leave one dead. Yet, it's just a statistic, which hardly changes our apathy towards road safety. Yes, road safety is an unpleasant, boring subject, but remember, it affects us all. How safe is the car you are driving or about to buy? How does one rate safety and who does it? We have compiled some information on a handful of cars that are available across the world and are also on sale in India, and their safety features and test results. We think you have the right to know. First, a little backgrounder. By law, all cars have to be tested and have to meet certain standards to be street legal. New Car Assessment Programmes (NCAPs) are carried out by various testing agencies in the United States, Europe, Japan and Australia amongst other countries. The European NCAP carries out the most stringent crash tests, as well as pedestrian and child safety tests vis-Ã  -vis other assessment programmes. Euro NCAP is a collaboration between governmental and automotive associations and is overseen by the FIA (Federation Internationale de l'Automobile). The money for these expensive tests comes from the EU, by which the widest possible range of models can be assessed. Euro NCAP carries out frontal offset, side impact and pole test and has a simple rating system that awards stars out of five. This rating system makes it easy to interpret safety standards in cars before people go out to buy them. Over and above this, manufacturers can go ahead and carry out further tests that are not required by legislation and choose to incorporate these additional safety features. Let's then see how the cars have fared now. The Hyundai Accent GLS got an adult occupant rating of 2.5 stars and a pedestrian test rating of 2 stars. The levels of chest injury in adult occupants was exceptionally high, and hence the Accent missed out a full third star. Moreover, this car was fitted with a driver side airbag. For cars sold in Europe, side airbags come as an option. Accents are also equipped with Isofix anchorages and are electronically assisted by ABS and EBD.